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专利名称：Identification and comparison of protein-protein interactions that occur in
populations and identification of inhibitorsof these interactors
发明人：Krishnan Nandabalan,Jonathan Marc
Rothberg,Meijia Yang,James RobertKnight,Theodore Samuel Kalbfleisch
申请号：US08/874825申请日：19970613公开号：US06057101A公开日：20000502
摘要：Methods are described for detecting protein-protein interactions, among twopopulations of proteins, each having a complexity of at least 1, 000. For example, proteinsare fused either to the DNA- binding domain of a transcriptional activator or to theactivation domain of a transcriptional activator. Two yeast strains, of the opposite matingtype and carrying one type each of the fusion proteins are mated together. Productiveinteractions between the two halves due to protein- protein interactions lead to thereconstitution of the transcriptional activator, which in turn leads to the activation of areporter gene containing a binding site for the DNA-binding domain. This analysis can becarried out for two or more populations of proteins. The differences in the genesencoding the proteins involved in the protein-protein interactions are characterized, thusleading to the identification of specific protein- protein interactions, and the genesencoding the interacting proteins, relevant to a particular tissue, stage or disease.
Furthermore, inhibitors that interfere with these protein-protein interactions areidentified by their ability to inactivate a reporter gene. The screening for such inhibitorscan be in a multiplexed format where a set of inhibitors will be screened against a libraryof interactors. Further, information-processing methods and systems are described.These methods and systems provide for identification of the genes coding for detectedinteracting proteins, for assembling a unified database of protein- protein interactiondata, and for processing this unified database to obtain protein interaction domain andprotein pathway information.
申请人：CURAGEN CORPORATION
代理机构：Pennie & Edmonds LLP
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